Chemical Name: 25-HYDROXYCHOLESTEROL
Molecular Formula: C27H46O2
Formula Weight: 402.65
CAS No.: 2140-46-7
A stock solution of 25-Hydroxycholesterol may be prepared in ethanol and dissolved in cell culture medium to give a final concentration of 1 mg/ml.
25-Hydroxycholesterol was used to treat mouse neuroblastoma cells to activate the ABCA1 signaling and in studies related to fatty acid metabolism
26-The lipid 25-hydroxycholesterol (25HC) is produced by immune cells in response to infection. We demonstrate that in addition to interfering with viral entry and replication, 25HC also amplifies the activation of immune cells and increases the production of immune mediators. Furthermore, we show that the presence of 25HC is actually harmful in the setting of infection with influenza because the production of these immune mediators leads to damage to the host. These observations might have particular relevance for understanding the mechanisms behind the high pathogenicity of several recently emerged influenza strains, including the H5N1 [bird flu," and might have implications for the protection of the host from these virulent strains.
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